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High-throughput genetic screening of meiotic commitment using fluorescence microscopy in Saccharomyces cerevisiae

Simple genetic screens in budding yeast have identified many conserved meiotic regulators. However, the identification of genes involved in specific steps of meiosis may require a more complex genetic screen that allows visualization of meiosis. Here, we describe a high-throughput protocol using flu...

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Detalles Bibliográficos
Autores principales: Gavade, Janardan N., Lacefield, Soni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619721/
https://www.ncbi.nlm.nih.gov/pubmed/36325582
http://dx.doi.org/10.1016/j.xpro.2022.101797