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High-throughput genetic screening of meiotic commitment using fluorescence microscopy in Saccharomyces cerevisiae
Simple genetic screens in budding yeast have identified many conserved meiotic regulators. However, the identification of genes involved in specific steps of meiosis may require a more complex genetic screen that allows visualization of meiosis. Here, we describe a high-throughput protocol using flu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619721/ https://www.ncbi.nlm.nih.gov/pubmed/36325582 http://dx.doi.org/10.1016/j.xpro.2022.101797 |