Protocol to isolate live single cells while retaining spatial information by combining cell photolabeling and FACS

Single-cell techniques have revolutionized biology; however, the required sample processing inherently implies the loss of spatial localization. Here, using an approach called photoconversion of areas to dissect micro-environments (PADME), we detail steps to isolate live single cells from a primary...

Descripción completa

Detalles Bibliográficos
Autores principales: Baldominos, Pilar, Barreiro, Olga, von Andrian, Ulrich, Sirera, Rafael, Montero-Llopis, Paula, Agudo, Judith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619724/
https://www.ncbi.nlm.nih.gov/pubmed/36325581
http://dx.doi.org/10.1016/j.xpro.2022.101795

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9619724/
https://www.ncbi.nlm.nih.gov/pubmed/36325581
http://dx.doi.org/10.1016/j.xpro.2022.101795

Ejemplares similares