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A PCR-free rapid protocol for one-pot construction of highly diverse genetic libraries
In vitro protein display methods can access extensive libraries (e.g., 10(12)–10(14)) and play an increasingly important role in protein engineering. However, the preparation of large libraries remains a laborious and time-consuming process. Here we report an efficient one-pot ligation & elongat...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9621413/ https://www.ncbi.nlm.nih.gov/pubmed/36315516 http://dx.doi.org/10.1371/journal.pone.0276338 |