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Fusion primer driven racket PCR: A novel tool for genome walking

The limitations of the current genome-walking strategies include strong background and cumbersome experimental processes. Herein, we report a genome-walking method, fusion primer-driven racket PCR (FPR-PCR), for the reliable retrieval of unknown flanking DNA sequences. Four sequence-specific primers...

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Detalles Bibliográficos
Autores principales: Pei, Jinfeng, Sun, Tianyi, Wang, Lingqin, Pan, Zhenkang, Guo, Xinyue, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623105/
https://www.ncbi.nlm.nih.gov/pubmed/36330444
http://dx.doi.org/10.3389/fgene.2022.969840