AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes–mediated transformation

Agrobacterium rhizogenes–mediated (ARM) transformation is an efficient and powerful tool to generate transgenic roots to study root-related biology. For loss-of-function studies, transgenic-root-induced indel mutations by CRISPR/Cas9 only with homozygous/biallelic mutagenesis can exhibit mutant phen...

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Detalles Bibliográficos
Autores principales: Liu, Shuang, Wang, Xiuyuan, Li, Qianqian, Peng, Wentao, Zhang, Zunmian, Chu, Pengfei, Guo, Shangjing, Fan, Yinglun, Lyu, Shanhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623429/
https://www.ncbi.nlm.nih.gov/pubmed/36330262
http://dx.doi.org/10.3389/fpls.2022.952428

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9623429/
https://www.ncbi.nlm.nih.gov/pubmed/36330262
http://dx.doi.org/10.3389/fpls.2022.952428

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