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Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription

RNA research and applications are underpinned by in vitro transcription (IVT), but RNA impurities resulting from the enzymatic reagents severely impede downstream applications. To improve the stability and purity of synthesized RNA, we have characterized a novel single-subunit RNA polymerase (RNAP)...

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Detalles Bibliográficos
Autores principales: Xia, Heng, Yu, Bingbing, Jiang, Yixin, Cheng, Rui, Lu, Xueling, Wu, Hui, Zhu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9624206/
https://www.ncbi.nlm.nih.gov/pubmed/36299232
http://dx.doi.org/10.1080/15476286.2022.2139113