DNA Shuffling of aprE Genes to Increase Fibrinolytic Activity and Thermostability

Four aprE genes encoding alkaline serine proteases from B. subtilis strains were used as template genes for family gene shuffling. Shuffled genes obtained by DNase I digestion followed by consecutive primerless and regular PCR reactions were ligated with pHY300PLK, an E. coli-Bacillus shuttle vector...

Descripción completa

Detalles Bibliográficos
Autores principales: Yao, Zhuang, Jeon, Hye Sung, Yoo, Ji Yeon, Kang, Yun Ji, Kim, Min Jae, Kim, Tae Jin, Kim, Jeong Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2022
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628911/
https://www.ncbi.nlm.nih.gov/pubmed/35484964
http://dx.doi.org/10.4014/jmb.2202.02017

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9628911/
https://www.ncbi.nlm.nih.gov/pubmed/35484964
http://dx.doi.org/10.4014/jmb.2202.02017

Ejemplares similares