Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems

In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry f...

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Autores principales: Qi, Qianqian, Zhang, Yutong, Xiong, Wei, Liu, Xingyu, Cui, Shuangyu, Ye, Xiaofang, Zhang, Kaisong, Tian, Tian, Xiang Zhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629044/
https://www.ncbi.nlm.nih.gov/pubmed/36382274
http://dx.doi.org/10.1039/d2sc02635j
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author Qi, Qianqian
Zhang, Yutong
Xiong, Wei
Liu, Xingyu
Cui, Shuangyu
Ye, Xiaofang
Zhang, Kaisong
Tian, Tian
Xiang Zhou,
author_facet Qi, Qianqian
Zhang, Yutong
Xiong, Wei
Liu, Xingyu
Cui, Shuangyu
Ye, Xiaofang
Zhang, Kaisong
Tian, Tian
Xiang Zhou,
author_sort Qi, Qianqian
collection PubMed
description In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished.
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spelling pubmed-96290442022-11-14 Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems Qi, Qianqian Zhang, Yutong Xiong, Wei Liu, Xingyu Cui, Shuangyu Ye, Xiaofang Zhang, Kaisong Tian, Tian Xiang Zhou, Chem Sci Chemistry In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished. The Royal Society of Chemistry 2022-10-20 /pmc/articles/PMC9629044/ /pubmed/36382274 http://dx.doi.org/10.1039/d2sc02635j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qi, Qianqian
Zhang, Yutong
Xiong, Wei
Liu, Xingyu
Cui, Shuangyu
Ye, Xiaofang
Zhang, Kaisong
Tian, Tian
Xiang Zhou,
Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title_full Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title_fullStr Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title_full_unstemmed Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title_short Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems
title_sort norbornene-tetrazine ligation chemistry for controlling rna-guided crispr systems
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9629044/
https://www.ncbi.nlm.nih.gov/pubmed/36382274
http://dx.doi.org/10.1039/d2sc02635j
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