Protocol for ex vivo competition and sequencing of mycobacterium isolated from infected guinea pigs

We describe steps for gDNA isolation from mycobacterium strains isolated from guinea pig lungs. We detail steps for infection of guinea pigs with Mycobacterium tuberculosis, followed by in vitro growth, gDNA isolation, and whole genome sequencing. We also describe an ex vivo competition experiment t...

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Detalles Bibliográficos
Autores principales: Naz, Saba, Dabral, Shruti, Kumar, Dhiraj, Nandicoori, Vinay Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630790/
https://www.ncbi.nlm.nih.gov/pubmed/36340884
http://dx.doi.org/10.1016/j.xpro.2022.101804

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630790/
https://www.ncbi.nlm.nih.gov/pubmed/36340884
http://dx.doi.org/10.1016/j.xpro.2022.101804

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