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Protocol for ex vivo competition and sequencing of mycobacterium isolated from infected guinea pigs
We describe steps for gDNA isolation from mycobacterium strains isolated from guinea pig lungs. We detail steps for infection of guinea pigs with Mycobacterium tuberculosis, followed by in vitro growth, gDNA isolation, and whole genome sequencing. We also describe an ex vivo competition experiment t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9630790/ https://www.ncbi.nlm.nih.gov/pubmed/36340884 http://dx.doi.org/10.1016/j.xpro.2022.101804 |