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The potential of 5′ nuclease PCR for detecting a single-base polymorphism inOrthopoxvirus

A fluorogenic 5′ nuclease PCR assay was evaluated for its ability to specifically detect and differentiate DNA of twoOrthopoxvirusspecies. A pair of consensus primers that target a DNA segment of theOrthopoxvirushaemagglutinin gene, and two oligonucleotide probes, each labelled with a different fluo...

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Detalles Bibliográficos
Autores principales: Ibrahim, M.S., Esposito, J.J., Jahrling, P.B., Lofts, R.S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press. 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9631612/
https://www.ncbi.nlm.nih.gov/pubmed/9160329
http://dx.doi.org/10.1006/mcpr.1996.0093