Absolute protein quantification using fluorescence measurements with FPCountR

This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on the generation of bespoke fluorescent protein (FP) calibrants, assays to determine protein concentra...

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Detalles Bibliográficos
Autores principales: Csibra, Eszter, Stan, Guy-Bart
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633595/
https://www.ncbi.nlm.nih.gov/pubmed/36329019
http://dx.doi.org/10.1038/s41467-022-34232-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9633595/
https://www.ncbi.nlm.nih.gov/pubmed/36329019
http://dx.doi.org/10.1038/s41467-022-34232-6

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