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Improved chemical and isotopic labeling of biomembranes in Bacillus subtilis by leveraging CRISPRi inhibition of beta-ketoacyl-ACP synthase (fabF)

Assessing the structure of living microbial cell membranes is a challenging analytical goal. The cell membrane is defined by its transverse structure, an approximately 5 nm-thick selectively permeable bilayer that serves many important cellular functions. Compositionally complex, dynamic, and organi...

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Detalles Bibliográficos
Autores principales: Nickels, Jonathan D., Bonifer, Kyle S., Tindall, Rachel R., Yahya, Ahmad, Tan, Luoxi, Do, Changwoo, Davison, Brian H., Elkins, James G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9634059/
https://www.ncbi.nlm.nih.gov/pubmed/36339713
http://dx.doi.org/10.3389/fmolb.2022.1011981