Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing

We present Light-Seq, an approach for multiplexed spatial indexing of intact biological samples using light-directed DNA barcoding in fixed cells and tissues followed by ex situ sequencing. Light-Seq combines spatially targeted, rapid photocrosslinking of DNA barcodes onto complementary DNAs in situ...

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Detalles Bibliográficos
Autores principales: Kishi, Jocelyn Y., Liu, Ninning, West, Emma R., Sheng, Kuanwei, Jordanides, Jack J., Serrata, Matthew, Cepko, Constance L., Saka, Sinem K., Yin, Peng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636025/
https://www.ncbi.nlm.nih.gov/pubmed/36216958
http://dx.doi.org/10.1038/s41592-022-01604-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636025/
https://www.ncbi.nlm.nih.gov/pubmed/36216958
http://dx.doi.org/10.1038/s41592-022-01604-1

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