Cargando…

Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples

We developed a procedure for extracting maximal amounts of high-quality RNA from low-biomass producing (autotrophic) bacteria for experiments where sample volume is limited. Large amounts of high-quality RNA for downstream analyses cannot be obtained using larger quantities of culture volume. The pe...

Descripción completa

Detalles Bibliográficos
Autores principales: Verbeelen, Tom, Van Houdt, Rob, Leys, Natalie, Ganigué, Ramon, Mastroleo, Felice
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636042/
https://www.ncbi.nlm.nih.gov/pubmed/36345334
http://dx.doi.org/10.1016/j.isci.2022.105311