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Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples
We developed a procedure for extracting maximal amounts of high-quality RNA from low-biomass producing (autotrophic) bacteria for experiments where sample volume is limited. Large amounts of high-quality RNA for downstream analyses cannot be obtained using larger quantities of culture volume. The pe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636042/ https://www.ncbi.nlm.nih.gov/pubmed/36345334 http://dx.doi.org/10.1016/j.isci.2022.105311 |