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Optimization of RNA extraction for bacterial whole transcriptome studies of low-biomass samples

We developed a procedure for extracting maximal amounts of high-quality RNA from low-biomass producing (autotrophic) bacteria for experiments where sample volume is limited. Large amounts of high-quality RNA for downstream analyses cannot be obtained using larger quantities of culture volume. The pe...

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Detalles Bibliográficos
Autores principales:	Verbeelen, Tom, Van Houdt, Rob, Leys, Natalie, Ganigué, Ramon, Mastroleo, Felice
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636042/ https://www.ncbi.nlm.nih.gov/pubmed/36345334 http://dx.doi.org/10.1016/j.isci.2022.105311

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