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Construction and characterisation of glycoprotein E and glycoprotein I deficient mutants of Australian strains of infectious laryngotracheitis virus using traditional and CRISPR/Cas9-assisted homologous recombination techniques

In alphaherpesviruses, glycoproteins E and I (gE and gI, respectively) form a heterodimer that facilitates cell-to-cell spread of virus. Using traditional homologous recombination techniques, as well as CRISPR/Cas9-assisted homologous recombination, we separately deleted gE and gI coding sequences f...

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Detalles Bibliográficos
Autores principales: Armat, Marzieh, Vaz, Paola K., Browning, Glenn F., Noormohammadi, Amir H., Hartley, Carol A., Devlin, Joanne M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636094/
https://www.ncbi.nlm.nih.gov/pubmed/36127475
http://dx.doi.org/10.1007/s11262-022-01933-5