Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import

Achieving CRISPR Cas9-based manipulation of mitochondrial DNA (mtDNA) has been a long-standing goal and would be of great relevance for disease modeling and for clinical applications. In this project, we aimed to deliver Cas9 into the mitochondria of human cells and analyzed Cas9-induced mtDNA cleav...

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Detalles Bibliográficos
Autores principales: Schmiderer, Ludwig, Yudovich, David, Oburoglu, Leal, Hjort, Martin, Larsson, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636205/
https://www.ncbi.nlm.nih.gov/pubmed/36333335
http://dx.doi.org/10.1038/s41598-022-21794-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9636205/
https://www.ncbi.nlm.nih.gov/pubmed/36333335
http://dx.doi.org/10.1038/s41598-022-21794-0

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