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Protocol for SARS-CoV-2 infection of kidney organoids derived from human pluripotent stem cells

This protocol presents the use of SARS-CoV-2 isolates to infect human kidney organoids, enabling exploration of the impact of SARS-CoV-2 infection in a human multicellular in vitro system. We detail steps to generate kidney organoids from human pluripotent stem cells (hPSCs) and emulate a diabetic m...

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Detalles Bibliográficos
Autores principales: Garreta, Elena, Moya-Rull, Daniel, Stanifer, Megan L., Monteil, Vanessa, Prado, Patricia, Marco, Andrés, Tarantino, Carolina, Gallo, Maria, Jonsson, Gustav, Hagelkruys, Astrid, Mirazimi, Ali, Boulant, Steeve, Penninger, Josef M., Montserrat, Nuria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9637521/
https://www.ncbi.nlm.nih.gov/pubmed/36595951
http://dx.doi.org/10.1016/j.xpro.2022.101872