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KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors

BACKGROUND: We aimed to examine the effects of repetitive cortical spreading depression on the responses of nociceptive trigeminal neurons with dural afferents and characterize the role of 5-HT(1B/1D) and opioid receptors. METHODS: Trigeminocervical complex neurons (n = 53) responsive to nociceptive...

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Autores principales: Supronsinchai, Weera, Hoffmann, Jan, Akerman, Simon, Goadsby, Peter J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638706/
https://www.ncbi.nlm.nih.gov/pubmed/35833238
http://dx.doi.org/10.1177/03331024221112998
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author Supronsinchai, Weera
Hoffmann, Jan
Akerman, Simon
Goadsby, Peter J
author_facet Supronsinchai, Weera
Hoffmann, Jan
Akerman, Simon
Goadsby, Peter J
author_sort Supronsinchai, Weera
collection PubMed
description BACKGROUND: We aimed to examine the effects of repetitive cortical spreading depression on the responses of nociceptive trigeminal neurons with dural afferents and characterize the role of 5-HT(1B/1D) and opioid receptors. METHODS: Trigeminocervical complex neurons (n = 53) responsive to nociceptive activation of the dura mater were studied in rats using electrophysiological techniques. RESULTS: A sub-population (n = 32) showed an average inhibition of dural-evoked responses of 65 ± 14% from baseline with cortical spreading depression. This response was reversed by the selective 5-HT(1B/1D) receptor antagonist, GR127935 (3 mg/kg; n = 6, iv), and a non-selective opioid receptor antagonist, naloxone (1.5 mg/kg; n = 6, iv), five minutes after injection. To determine the role of the nucleus raphe magnus in the trigeminocervical complex inhibitory effect, microinjection of lidocaine (2%, n = 6) or muscimol (100 mM, n = 5) into the nucleus raphe magnus was performed. There was no effect on cortical spreading depression-induced inhibition of neuronal firing in trigeminocervical complex by either. CONCLUSION: The data demonstrate that repetitive cortical spreading depression inhibits a subpopulation of dural nociceptive trigeminocervical neurons, an effect mediated by serotonin and opioid receptors. This inhibition does not involve modulation of nucleus raphe magnus neurons.
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spelling pubmed-96387062022-11-08 KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors Supronsinchai, Weera Hoffmann, Jan Akerman, Simon Goadsby, Peter J Cephalalgia Original Articles BACKGROUND: We aimed to examine the effects of repetitive cortical spreading depression on the responses of nociceptive trigeminal neurons with dural afferents and characterize the role of 5-HT(1B/1D) and opioid receptors. METHODS: Trigeminocervical complex neurons (n = 53) responsive to nociceptive activation of the dura mater were studied in rats using electrophysiological techniques. RESULTS: A sub-population (n = 32) showed an average inhibition of dural-evoked responses of 65 ± 14% from baseline with cortical spreading depression. This response was reversed by the selective 5-HT(1B/1D) receptor antagonist, GR127935 (3 mg/kg; n = 6, iv), and a non-selective opioid receptor antagonist, naloxone (1.5 mg/kg; n = 6, iv), five minutes after injection. To determine the role of the nucleus raphe magnus in the trigeminocervical complex inhibitory effect, microinjection of lidocaine (2%, n = 6) or muscimol (100 mM, n = 5) into the nucleus raphe magnus was performed. There was no effect on cortical spreading depression-induced inhibition of neuronal firing in trigeminocervical complex by either. CONCLUSION: The data demonstrate that repetitive cortical spreading depression inhibits a subpopulation of dural nociceptive trigeminocervical neurons, an effect mediated by serotonin and opioid receptors. This inhibition does not involve modulation of nucleus raphe magnus neurons. SAGE Publications 2022-07-13 2022-11 /pmc/articles/PMC9638706/ /pubmed/35833238 http://dx.doi.org/10.1177/03331024221112998 Text en © International Headache Society 2022 https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Supronsinchai, Weera
Hoffmann, Jan
Akerman, Simon
Goadsby, Peter J
KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title_full KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title_fullStr KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title_full_unstemmed KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title_short KCl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-HT(1B/1D) and opioid receptors
title_sort kcl-induced repetitive cortical spreading depression inhibiting trigeminal neuronal firing is mediated by 5-ht(1b/1d) and opioid receptors
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638706/
https://www.ncbi.nlm.nih.gov/pubmed/35833238
http://dx.doi.org/10.1177/03331024221112998
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