Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients

Utilizing chemically synthesized an isotopically labeled internal standard, isodesmosine-(13)C(3),(15)N(1), an isotope-dilution LC-MS/MS method was established. Concentrations of desmosine and isodesmosine in plasma of acute cerebral stroke patients and healthy controls were determined. The concentr...

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Autores principales: Mikagi, Ayame, Tashiro, Ryosuke, Inoue, Tomoo, Anzawa, Riki, Imura, Akiho, Tanigawa, Takahiro, Ishida, Tomohisa, Inoue, Takashi, Niizuma, Kuniyasu, Tominaga, Teiji, Usuki, Toyonobu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639221/
https://www.ncbi.nlm.nih.gov/pubmed/36380946
http://dx.doi.org/10.1039/d2ra06009d
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author Mikagi, Ayame
Tashiro, Ryosuke
Inoue, Tomoo
Anzawa, Riki
Imura, Akiho
Tanigawa, Takahiro
Ishida, Tomohisa
Inoue, Takashi
Niizuma, Kuniyasu
Tominaga, Teiji
Usuki, Toyonobu
author_facet Mikagi, Ayame
Tashiro, Ryosuke
Inoue, Tomoo
Anzawa, Riki
Imura, Akiho
Tanigawa, Takahiro
Ishida, Tomohisa
Inoue, Takashi
Niizuma, Kuniyasu
Tominaga, Teiji
Usuki, Toyonobu
author_sort Mikagi, Ayame
collection PubMed
description Utilizing chemically synthesized an isotopically labeled internal standard, isodesmosine-(13)C(3),(15)N(1), an isotope-dilution LC-MS/MS method was established. Concentrations of desmosine and isodesmosine in plasma of acute cerebral stroke patients and healthy controls were determined. The concentration of desmosines was markedly higher in plasma from acute stroke patients compared with healthy controls. Desmosines are thus novel biomarkers for evaluating the extent of vascular injury after acute cerebral stroke.
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spelling pubmed-96392212022-11-14 Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients Mikagi, Ayame Tashiro, Ryosuke Inoue, Tomoo Anzawa, Riki Imura, Akiho Tanigawa, Takahiro Ishida, Tomohisa Inoue, Takashi Niizuma, Kuniyasu Tominaga, Teiji Usuki, Toyonobu RSC Adv Chemistry Utilizing chemically synthesized an isotopically labeled internal standard, isodesmosine-(13)C(3),(15)N(1), an isotope-dilution LC-MS/MS method was established. Concentrations of desmosine and isodesmosine in plasma of acute cerebral stroke patients and healthy controls were determined. The concentration of desmosines was markedly higher in plasma from acute stroke patients compared with healthy controls. Desmosines are thus novel biomarkers for evaluating the extent of vascular injury after acute cerebral stroke. The Royal Society of Chemistry 2022-11-07 /pmc/articles/PMC9639221/ /pubmed/36380946 http://dx.doi.org/10.1039/d2ra06009d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mikagi, Ayame
Tashiro, Ryosuke
Inoue, Tomoo
Anzawa, Riki
Imura, Akiho
Tanigawa, Takahiro
Ishida, Tomohisa
Inoue, Takashi
Niizuma, Kuniyasu
Tominaga, Teiji
Usuki, Toyonobu
Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title_full Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title_fullStr Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title_full_unstemmed Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title_short Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
title_sort isotope-dilution lc-ms/ms analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9639221/
https://www.ncbi.nlm.nih.gov/pubmed/36380946
http://dx.doi.org/10.1039/d2ra06009d
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