An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells

We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo c...

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Autores principales: Surendran, Harshini, Soundararajan, Lalitha, Reddy K, Vijay Bhaskar, Subramani, Janavi, Stoddard, Jonathan, Reynaga, Rene, Tschetter, Wayne, Ryals, Renee C., Pal, Rajarshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641055/
https://www.ncbi.nlm.nih.gov/pubmed/36386870
http://dx.doi.org/10.1016/j.xpro.2022.101803
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author Surendran, Harshini
Soundararajan, Lalitha
Reddy K, Vijay Bhaskar
Subramani, Janavi
Stoddard, Jonathan
Reynaga, Rene
Tschetter, Wayne
Ryals, Renee C.
Pal, Rajarshi
author_facet Surendran, Harshini
Soundararajan, Lalitha
Reddy K, Vijay Bhaskar
Subramani, Janavi
Stoddard, Jonathan
Reynaga, Rene
Tschetter, Wayne
Ryals, Renee C.
Pal, Rajarshi
author_sort Surendran, Harshini
collection PubMed
description We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021).
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spelling pubmed-96410552022-11-15 An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells Surendran, Harshini Soundararajan, Lalitha Reddy K, Vijay Bhaskar Subramani, Janavi Stoddard, Jonathan Reynaga, Rene Tschetter, Wayne Ryals, Renee C. Pal, Rajarshi STAR Protoc Protocol We present an optimized protocol for guided differentiation of retinal pigment epithelium (RPE) cells from human-induced pluripotent stem cells (iPSC). De novo-generated RPE cells are mature, polarized, and mimic the cellular and molecular profile of primary RPE; they are also suitable for in vivo cell transplantation studies. The protocol includes an enrichment step, making it useful for large-scale GMP manufacturing. RPE cells produced following this protocol are appropriate for cell replacement therapy for macular degeneration and disease modeling. For complete details on the use and execution of this protocol, please refer to Surendran et al. (2021). Elsevier 2022-11-04 /pmc/articles/PMC9641055/ /pubmed/36386870 http://dx.doi.org/10.1016/j.xpro.2022.101803 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Surendran, Harshini
Soundararajan, Lalitha
Reddy K, Vijay Bhaskar
Subramani, Janavi
Stoddard, Jonathan
Reynaga, Rene
Tschetter, Wayne
Ryals, Renee C.
Pal, Rajarshi
An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title_full An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title_fullStr An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title_full_unstemmed An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title_short An improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
title_sort improved protocol for generation and characterization of human-induced pluripotent stem cell-derived retinal pigment epithelium cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641055/
https://www.ncbi.nlm.nih.gov/pubmed/36386870
http://dx.doi.org/10.1016/j.xpro.2022.101803
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