Protocol to image and quantify nucleocytoplasmic transport in cultured cells using fluorescent in situ hybridization and a dual reporter system

Nucleocytoplasmic transport (NCT) plays critical roles in maintaining cellular homeostasis. Here, we present a protocol to measure NCT for both transcript and protein cargos in cultured cells. We first describe the fluorescent in situ hybridization (FISH) assay to measure the nuclear mRNA export. We...

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Detalles Bibliográficos
Autores principales: Cui, Haochen, Sepehrimanesh, Masood, Coutee, Casey A., Akter, Masuma, Hosain, Md Abir, Ding, Baojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641092/
https://www.ncbi.nlm.nih.gov/pubmed/36386872
http://dx.doi.org/10.1016/j.xpro.2022.101813

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9641092/
https://www.ncbi.nlm.nih.gov/pubmed/36386872
http://dx.doi.org/10.1016/j.xpro.2022.101813

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