Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells
Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype due to its greater invasive capacity and non-response to hormone therapy. Several species of the Ficus genus have been used as an alternative to traditional medicine against malignant diseases. Previously, leaf extract...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647462/ https://www.ncbi.nlm.nih.gov/pubmed/36387436 http://dx.doi.org/10.1016/j.heliyon.2022.e11405 |
_version_ | 1784827388639576064 |
---|---|
author | Cayetano-Salazar, Lorena de la Cruz-Concepción, Brenda Navarro-Tito, Napoleón Álvarez-Fitz, Patricia Leyva-Vázquez, Marco A. Acevedo-Quiroz, Macdiel Zacapala-Gómez, Ana E. Ortuño-Pineda, Carlos Martinez-Carrillo, Dinorah N. Castañeda-Saucedo, Eduardo García-Hernández, Alejandra P. Mendoza-Catalán, Miguel A. |
author_facet | Cayetano-Salazar, Lorena de la Cruz-Concepción, Brenda Navarro-Tito, Napoleón Álvarez-Fitz, Patricia Leyva-Vázquez, Marco A. Acevedo-Quiroz, Macdiel Zacapala-Gómez, Ana E. Ortuño-Pineda, Carlos Martinez-Carrillo, Dinorah N. Castañeda-Saucedo, Eduardo García-Hernández, Alejandra P. Mendoza-Catalán, Miguel A. |
author_sort | Cayetano-Salazar, Lorena |
collection | PubMed |
description | Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype due to its greater invasive capacity and non-response to hormone therapy. Several species of the Ficus genus have been used as an alternative to traditional medicine against malignant diseases. Previously, leaf extracts from Ficus crocata (Miq.) Mart. ex Miq. (F. crocata) showed antiproliferative activity in vitro against breast and cervical tumor cells without having a cytotoxic effect on non-tumor cell lines. The purpose of the study was to evaluate the effect of hexane (Hex-EFc), dichloromethane (Dic-EFc), and acetone (Ace-EFc) extracts from F. crocata on the proliferative and invasive capacity of breast cancer cells MCF-7 and MDA-MB-231. MATERIALS AND METHODS: The phytochemical profile was carried out by gas chromatography-mass spectrometry (GC-MS). Cell proliferation, migration, and invasion were determined by MTT, wound closure, and transwell assays, respectively. MMPs activity was analyzed using gelatin zymography, and fluorescence microscopy was used to visualize F-actin distribution. RESULTS: Hex-EFc, Dic-EFc, and Ace-EFc showed cytotoxic activity on MDA-MB-231 tumor cells and, to a lesser extent, on MCF-7 cells, without presenting cytotoxicity at the same concentrations in MCF-10A non-tumor cells. Dic-EFc and Ace-EFc (5–10 μg/mL) reduced the migration capacity of MCF-7 and MDA-MB-231 cells. Interestingly, exposure to Dic-EFc and Ace-EFc (5–10 μg/mL) inhibited the invasive ability of MDA-MB-231 cells, reducing the secretion and activity of MMP-2 and MMP-9, as well as the F-actin distribution. CONCLUSIONS: Dic-EFc and Ace-EFc at low concentrations decreased breast cancer cell proliferation and invasiveness, mainly of MDA-MB-231 cells. The above supports the potential use of compounds from leaf extracts of F. crocata in neoadjuvant therapy to reduce the progression of breast cancer tumors, mainly triple-negative tumors. |
format | Online Article Text |
id | pubmed-9647462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-96474622022-11-15 Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells Cayetano-Salazar, Lorena de la Cruz-Concepción, Brenda Navarro-Tito, Napoleón Álvarez-Fitz, Patricia Leyva-Vázquez, Marco A. Acevedo-Quiroz, Macdiel Zacapala-Gómez, Ana E. Ortuño-Pineda, Carlos Martinez-Carrillo, Dinorah N. Castañeda-Saucedo, Eduardo García-Hernández, Alejandra P. Mendoza-Catalán, Miguel A. Heliyon Research Article Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype due to its greater invasive capacity and non-response to hormone therapy. Several species of the Ficus genus have been used as an alternative to traditional medicine against malignant diseases. Previously, leaf extracts from Ficus crocata (Miq.) Mart. ex Miq. (F. crocata) showed antiproliferative activity in vitro against breast and cervical tumor cells without having a cytotoxic effect on non-tumor cell lines. The purpose of the study was to evaluate the effect of hexane (Hex-EFc), dichloromethane (Dic-EFc), and acetone (Ace-EFc) extracts from F. crocata on the proliferative and invasive capacity of breast cancer cells MCF-7 and MDA-MB-231. MATERIALS AND METHODS: The phytochemical profile was carried out by gas chromatography-mass spectrometry (GC-MS). Cell proliferation, migration, and invasion were determined by MTT, wound closure, and transwell assays, respectively. MMPs activity was analyzed using gelatin zymography, and fluorescence microscopy was used to visualize F-actin distribution. RESULTS: Hex-EFc, Dic-EFc, and Ace-EFc showed cytotoxic activity on MDA-MB-231 tumor cells and, to a lesser extent, on MCF-7 cells, without presenting cytotoxicity at the same concentrations in MCF-10A non-tumor cells. Dic-EFc and Ace-EFc (5–10 μg/mL) reduced the migration capacity of MCF-7 and MDA-MB-231 cells. Interestingly, exposure to Dic-EFc and Ace-EFc (5–10 μg/mL) inhibited the invasive ability of MDA-MB-231 cells, reducing the secretion and activity of MMP-2 and MMP-9, as well as the F-actin distribution. CONCLUSIONS: Dic-EFc and Ace-EFc at low concentrations decreased breast cancer cell proliferation and invasiveness, mainly of MDA-MB-231 cells. The above supports the potential use of compounds from leaf extracts of F. crocata in neoadjuvant therapy to reduce the progression of breast cancer tumors, mainly triple-negative tumors. Elsevier 2022-11-05 /pmc/articles/PMC9647462/ /pubmed/36387436 http://dx.doi.org/10.1016/j.heliyon.2022.e11405 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Cayetano-Salazar, Lorena de la Cruz-Concepción, Brenda Navarro-Tito, Napoleón Álvarez-Fitz, Patricia Leyva-Vázquez, Marco A. Acevedo-Quiroz, Macdiel Zacapala-Gómez, Ana E. Ortuño-Pineda, Carlos Martinez-Carrillo, Dinorah N. Castañeda-Saucedo, Eduardo García-Hernández, Alejandra P. Mendoza-Catalán, Miguel A. Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title | Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title_full | Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title_fullStr | Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title_full_unstemmed | Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title_short | Ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
title_sort | ficus crocata leaf extracts decrease the proliferation and invasiveness of breast cancer cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647462/ https://www.ncbi.nlm.nih.gov/pubmed/36387436 http://dx.doi.org/10.1016/j.heliyon.2022.e11405 |
work_keys_str_mv | AT cayetanosalazarlorena ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT delacruzconcepcionbrenda ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT navarrotitonapoleon ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT alvarezfitzpatricia ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT leyvavazquezmarcoa ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT acevedoquirozmacdiel ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT zacapalagomezanae ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT ortunopinedacarlos ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT martinezcarrillodinorahn ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT castanedasaucedoeduardo ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT garciahernandezalejandrap ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells AT mendozacatalanmiguela ficuscrocataleafextractsdecreasetheproliferationandinvasivenessofbreastcancercells |