Protocol to obtain high-quality single-cell RNA-sequencing data from mouse liver cells using centrifugation

High-quality single-cell RNA-sequencing data of liver cells, especially hepatocytes, are challenging due to cell death associated with hepatocyte isolation using fluorescence-activated cell sorting (FACS). Here, we present a protocol to obtain viable hepatocytes and nonparenchymal liver cells for sc...

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Detalles Bibliográficos
Autor principal: Wang, Simeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647703/
https://www.ncbi.nlm.nih.gov/pubmed/36386875
http://dx.doi.org/10.1016/j.xpro.2022.101824

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647703/
https://www.ncbi.nlm.nih.gov/pubmed/36386875
http://dx.doi.org/10.1016/j.xpro.2022.101824

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