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Protocol to obtain high-quality single-cell RNA-sequencing data from mouse liver cells using centrifugation
High-quality single-cell RNA-sequencing data of liver cells, especially hepatocytes, are challenging due to cell death associated with hepatocyte isolation using fluorescence-activated cell sorting (FACS). Here, we present a protocol to obtain viable hepatocytes and nonparenchymal liver cells for sc...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9647703/ https://www.ncbi.nlm.nih.gov/pubmed/36386875 http://dx.doi.org/10.1016/j.xpro.2022.101824 |