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Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage

Probiotics are typically enumerated by agar plate counting (PC) techniques. PC has several limitations including poor specificity, high variability, inability to enumerate dead cells, viable but non-culturable cells and cells in complex matrices. Viability droplet digital polymerase chain reaction (...

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Detalles Bibliográficos
Autores principales: Kiefer, Anthony, Byrd, Phillip M., Tang, Peipei, Jones, Gregory, Galles, Kevin, Fallico, Vincenzo, Wong, Connie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648155/
https://www.ncbi.nlm.nih.gov/pubmed/36386724
http://dx.doi.org/10.3389/fmicb.2022.966264