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Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage
Probiotics are typically enumerated by agar plate counting (PC) techniques. PC has several limitations including poor specificity, high variability, inability to enumerate dead cells, viable but non-culturable cells and cells in complex matrices. Viability droplet digital polymerase chain reaction (...
Autores principales: | Kiefer, Anthony, Byrd, Phillip M., Tang, Peipei, Jones, Gregory, Galles, Kevin, Fallico, Vincenzo, Wong, Connie |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648155/ https://www.ncbi.nlm.nih.gov/pubmed/36386724 http://dx.doi.org/10.3389/fmicb.2022.966264 |
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