Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans

Transcriptional adaptation is a powerful gene regulation mechanism that can increase genetic robustness. Transcriptional adaptation occurs when a gene is mutated and is mediated by the mutant RNA, rather than by protein feedback loops. We show here that transcriptional adaptation occurs in the C. el...

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Autores principales: Fernandez-Abascal, Jesus, Wang, Lei, Graziano, Bianca, Johnson, Christina K., Bianchi, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648848/
https://www.ncbi.nlm.nih.gov/pubmed/36315586
http://dx.doi.org/10.1371/journal.pgen.1010488
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author Fernandez-Abascal, Jesus
Wang, Lei
Graziano, Bianca
Johnson, Christina K.
Bianchi, Laura
author_facet Fernandez-Abascal, Jesus
Wang, Lei
Graziano, Bianca
Johnson, Christina K.
Bianchi, Laura
author_sort Fernandez-Abascal, Jesus
collection PubMed
description Transcriptional adaptation is a powerful gene regulation mechanism that can increase genetic robustness. Transcriptional adaptation occurs when a gene is mutated and is mediated by the mutant RNA, rather than by protein feedback loops. We show here that transcriptional adaptation occurs in the C. elegans clh family of Cl(-) channels and that it requires exon-junction complex (EJC) proteins RNP-4, MAG-1, and eiF4AIII. Depending on which exons are deleted in distinct clh-1 alleles, different clh genes are regulated in an EJC-dependent manner. Our results support the idea that different transcriptional adaptation outcomes may be directed by the differential interaction of the EJC with its target mutant RNAs.
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spelling pubmed-96488482022-11-15 Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans Fernandez-Abascal, Jesus Wang, Lei Graziano, Bianca Johnson, Christina K. Bianchi, Laura PLoS Genet Research Article Transcriptional adaptation is a powerful gene regulation mechanism that can increase genetic robustness. Transcriptional adaptation occurs when a gene is mutated and is mediated by the mutant RNA, rather than by protein feedback loops. We show here that transcriptional adaptation occurs in the C. elegans clh family of Cl(-) channels and that it requires exon-junction complex (EJC) proteins RNP-4, MAG-1, and eiF4AIII. Depending on which exons are deleted in distinct clh-1 alleles, different clh genes are regulated in an EJC-dependent manner. Our results support the idea that different transcriptional adaptation outcomes may be directed by the differential interaction of the EJC with its target mutant RNAs. Public Library of Science 2022-10-31 /pmc/articles/PMC9648848/ /pubmed/36315586 http://dx.doi.org/10.1371/journal.pgen.1010488 Text en © 2022 Fernandez-Abascal et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fernandez-Abascal, Jesus
Wang, Lei
Graziano, Bianca
Johnson, Christina K.
Bianchi, Laura
Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title_full Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title_fullStr Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title_full_unstemmed Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title_short Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans
title_sort exon-dependent transcriptional adaptation by exon-junction complex proteins y14/rnp-4 and magoh/mag-1 in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9648848/
https://www.ncbi.nlm.nih.gov/pubmed/36315586
http://dx.doi.org/10.1371/journal.pgen.1010488
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