Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs

The high occurrence rate and difficulties in symptom control are listed as the major problems of oral mucosal disease by medical professionals. Following the development of oral mucosal lesions, the oral microenvironment changes, immunity declines, and continuous bacterial stimulation causes wound i...

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Autores principales: Qi, Wenxin, Dong, Naijun, Wu, Lingling, Zhang, Xueqi, Li, He, Wu, Hao, Ward, Natalie, Yu, Jian, Liu, He, Wang, Jiao, Deng, Xiaoyong, Zhao, Robert Chunhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650008/
https://www.ncbi.nlm.nih.gov/pubmed/36406253
http://dx.doi.org/10.1016/j.bioactmat.2022.10.027
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author Qi, Wenxin
Dong, Naijun
Wu, Lingling
Zhang, Xueqi
Li, He
Wu, Hao
Ward, Natalie
Yu, Jian
Liu, He
Wang, Jiao
Deng, Xiaoyong
Zhao, Robert Chunhua
author_facet Qi, Wenxin
Dong, Naijun
Wu, Lingling
Zhang, Xueqi
Li, He
Wu, Hao
Ward, Natalie
Yu, Jian
Liu, He
Wang, Jiao
Deng, Xiaoyong
Zhao, Robert Chunhua
author_sort Qi, Wenxin
collection PubMed
description The high occurrence rate and difficulties in symptom control are listed as the major problems of oral mucosal disease by medical professionals. Following the development of oral mucosal lesions, the oral microenvironment changes, immunity declines, and continuous bacterial stimulation causes wound infection. Traditional antibacterial drugs are ineffective for oral mucosal lesions. To overcome this problem, a light-responsive antibacterial hydrogel containing sustained-release BMSCs was inspired by the trauma environment in the oral cavity, which is different from that on the body surface since it mostly remains under dark conditions. In the absence of light, the hydrogel seals the wound to form a barrier, exerts a natural bacteriostatic effect, and prevents invasion by foreign bacteria. Simultaneously, mesenchymal stem cells are presented, and the released growth factors and other substances have excellent anti-inflammatory and angiogenic effects, which result in rapid repair of the damaged site. Under light conditions, after photo-induced shedding of the hydrogel, RuB(2)A exerts an antibacterial effect accompanied by degradation of the hydrogel. Results in a rat oral mucosal repair model demonstrate that DCS-RuB(2)A(2)-BMSCs could rapidly repair the oral mucosa within 4 days. Sequencing data provide ideas for further analysis of the intrinsic molecular mechanisms and signaling pathways. Taken together, our results suggest that this light-responsive antibacterial hydrogel loaded with BMSCs can be used for rapid wound repair and may advance the development of therapeutic strategies for the treatment of clinical oral mucosal defects.
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spelling pubmed-96500082022-11-18 Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs Qi, Wenxin Dong, Naijun Wu, Lingling Zhang, Xueqi Li, He Wu, Hao Ward, Natalie Yu, Jian Liu, He Wang, Jiao Deng, Xiaoyong Zhao, Robert Chunhua Bioact Mater Article The high occurrence rate and difficulties in symptom control are listed as the major problems of oral mucosal disease by medical professionals. Following the development of oral mucosal lesions, the oral microenvironment changes, immunity declines, and continuous bacterial stimulation causes wound infection. Traditional antibacterial drugs are ineffective for oral mucosal lesions. To overcome this problem, a light-responsive antibacterial hydrogel containing sustained-release BMSCs was inspired by the trauma environment in the oral cavity, which is different from that on the body surface since it mostly remains under dark conditions. In the absence of light, the hydrogel seals the wound to form a barrier, exerts a natural bacteriostatic effect, and prevents invasion by foreign bacteria. Simultaneously, mesenchymal stem cells are presented, and the released growth factors and other substances have excellent anti-inflammatory and angiogenic effects, which result in rapid repair of the damaged site. Under light conditions, after photo-induced shedding of the hydrogel, RuB(2)A exerts an antibacterial effect accompanied by degradation of the hydrogel. Results in a rat oral mucosal repair model demonstrate that DCS-RuB(2)A(2)-BMSCs could rapidly repair the oral mucosa within 4 days. Sequencing data provide ideas for further analysis of the intrinsic molecular mechanisms and signaling pathways. Taken together, our results suggest that this light-responsive antibacterial hydrogel loaded with BMSCs can be used for rapid wound repair and may advance the development of therapeutic strategies for the treatment of clinical oral mucosal defects. KeAi Publishing 2022-11-09 /pmc/articles/PMC9650008/ /pubmed/36406253 http://dx.doi.org/10.1016/j.bioactmat.2022.10.027 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Qi, Wenxin
Dong, Naijun
Wu, Lingling
Zhang, Xueqi
Li, He
Wu, Hao
Ward, Natalie
Yu, Jian
Liu, He
Wang, Jiao
Deng, Xiaoyong
Zhao, Robert Chunhua
Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title_full Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title_fullStr Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title_full_unstemmed Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title_short Promoting oral mucosal wound healing using a DCS-RuB(2)A(2) hydrogel based on a photoreactive antibacterial and sustained release of BMSCs
title_sort promoting oral mucosal wound healing using a dcs-rub(2)a(2) hydrogel based on a photoreactive antibacterial and sustained release of bmscs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9650008/
https://www.ncbi.nlm.nih.gov/pubmed/36406253
http://dx.doi.org/10.1016/j.bioactmat.2022.10.027
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