Immobilization of Penicillin G Acylase on Vinyl Sulfone-Agarose: An Unexpected Effect of the Ionic Strength on the Performance of the Immobilization Process

Penicillin G acylase (PGA) from Escherichia coli was immobilized on vinyl sulfone (VS) agarose. The immobilization of the enzyme failed at all pH values using 50 mM of buffer, while the progressive increase of ionic strength permitted its rapid immobilization under all studied pH values. This sugges...

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Detalles Bibliográficos
Autores principales: da Rocha, Thays N., Morellon-Sterlling, Roberto, Rocha-Martin, Javier, Bolivar, Juan M., Gonçalves, Luciana R. B., Fernandez-Lafuente, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654356/
https://www.ncbi.nlm.nih.gov/pubmed/36364414
http://dx.doi.org/10.3390/molecules27217587

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9654356/
https://www.ncbi.nlm.nih.gov/pubmed/36364414
http://dx.doi.org/10.3390/molecules27217587

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