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The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures

Despite the lack of scientific evidence, some consumers assert that raw milk is a natural food with nutritional and immunological properties superior to pasteurized milk. This has led to the increased popularity of unpasteurized cow milk (UPM) and disregard for the risks of being exposed to zoonotic...

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Autores principales: Idland, Lene, Bø-Granquist, Erik G., Aspholm, Marina, Lindbäck, Toril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656703/
https://www.ncbi.nlm.nih.gov/pubmed/36360022
http://dx.doi.org/10.3390/foods11213411
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author Idland, Lene
Bø-Granquist, Erik G.
Aspholm, Marina
Lindbäck, Toril
author_facet Idland, Lene
Bø-Granquist, Erik G.
Aspholm, Marina
Lindbäck, Toril
author_sort Idland, Lene
collection PubMed
description Despite the lack of scientific evidence, some consumers assert that raw milk is a natural food with nutritional and immunological properties superior to pasteurized milk. This has led to the increased popularity of unpasteurized cow milk (UPM) and disregard for the risks of being exposed to zoonotic infections. Dairy cattle are healthy carriers of Shiga toxin (Stx)-producing E. coli (STEC), and contaminated UPM has caused STEC outbreaks worldwide. The association between STEC, carrying the eae (E. coli attachment effacement) gene, and severe diseases is well-established. We have previously isolated four eae positive STEC isolates from two neighboring dairy farms in the Southeast of Norway. A whole genome analysis revealed that isolates from different farms exhibited nearly identical genetic profiles. To explore the risks associated with drinking UPM, we examined the ability of the isolates to produce Stx and their growth in UPM at different temperatures. All the isolates produced Stx and one of the isolates was able to propagate in UPM at 8 °C (p < 0.02). Altogether, these results highlight the risk for STEC infections associated with the consumption of UPM.
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spelling pubmed-96567032022-11-15 The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures Idland, Lene Bø-Granquist, Erik G. Aspholm, Marina Lindbäck, Toril Foods Article Despite the lack of scientific evidence, some consumers assert that raw milk is a natural food with nutritional and immunological properties superior to pasteurized milk. This has led to the increased popularity of unpasteurized cow milk (UPM) and disregard for the risks of being exposed to zoonotic infections. Dairy cattle are healthy carriers of Shiga toxin (Stx)-producing E. coli (STEC), and contaminated UPM has caused STEC outbreaks worldwide. The association between STEC, carrying the eae (E. coli attachment effacement) gene, and severe diseases is well-established. We have previously isolated four eae positive STEC isolates from two neighboring dairy farms in the Southeast of Norway. A whole genome analysis revealed that isolates from different farms exhibited nearly identical genetic profiles. To explore the risks associated with drinking UPM, we examined the ability of the isolates to produce Stx and their growth in UPM at different temperatures. All the isolates produced Stx and one of the isolates was able to propagate in UPM at 8 °C (p < 0.02). Altogether, these results highlight the risk for STEC infections associated with the consumption of UPM. MDPI 2022-10-28 /pmc/articles/PMC9656703/ /pubmed/36360022 http://dx.doi.org/10.3390/foods11213411 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Idland, Lene
Bø-Granquist, Erik G.
Aspholm, Marina
Lindbäck, Toril
The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title_full The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title_fullStr The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title_full_unstemmed The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title_short The Ability of Shiga Toxin-Producing Escherichia coli to Grow in Raw Cow’s Milk Stored at Low Temperatures
title_sort ability of shiga toxin-producing escherichia coli to grow in raw cow’s milk stored at low temperatures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9656703/
https://www.ncbi.nlm.nih.gov/pubmed/36360022
http://dx.doi.org/10.3390/foods11213411
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