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A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability

Proanthocyanidins (PACs) are a group of bioactive molecules found in a variety of plants and foods. Their bioavailability depends on their molecular size, with monomers and dimers being more bioavailable than those that have a higher polymerization degree. This study aimed to develop a method to con...

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Autores principales: Iannuzzo, Fortuna, Piccolo, Vincenzo, Novellino, Ettore, Schiano, Elisabetta, Salviati, Emanuela, Summa, Vincenzo, Campiglia, Pietro, Tenore, Gian Carlo, Maisto, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657391/
https://www.ncbi.nlm.nih.gov/pubmed/36362344
http://dx.doi.org/10.3390/ijms232113557
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author Iannuzzo, Fortuna
Piccolo, Vincenzo
Novellino, Ettore
Schiano, Elisabetta
Salviati, Emanuela
Summa, Vincenzo
Campiglia, Pietro
Tenore, Gian Carlo
Maisto, Maria
author_facet Iannuzzo, Fortuna
Piccolo, Vincenzo
Novellino, Ettore
Schiano, Elisabetta
Salviati, Emanuela
Summa, Vincenzo
Campiglia, Pietro
Tenore, Gian Carlo
Maisto, Maria
author_sort Iannuzzo, Fortuna
collection PubMed
description Proanthocyanidins (PACs) are a group of bioactive molecules found in a variety of plants and foods. Their bioavailability depends on their molecular size, with monomers and dimers being more bioavailable than those that have a higher polymerization degree. This study aimed to develop a method to convert high-molecular-weight PACs to low-molecular-weight ones in a grape seed extract (GSE) from Vitis vinifera L. Therefore, GSE was subjected to alkaline treatment (ATGSE), and its difference in chemical composition, compared to GSE, was evaluated using a molecular networking (MN) approach based on results obtained from HPLC-ESI HRMS/MS characterization analysis. The network analysis mainly noted the PAC cluster with about 142 PAC compounds identified. In particular, the obtained results showed a higher content of monomeric and dimeric PACs in ATGSE compared to GSE, with 58% and 49% monomers and 31% and 24% dimers, respectively. Conversely, trimeric (9%), polymeric (4%), and galloylated PACs (14%) were more abundant in GSE than in ATGSE (6%, 1%, and 4%, respectively). Moreover, in vitro antioxidant and anti-inflammatory activities were investigated, showing the high beneficial potential of both extracts. In conclusion, ATGSE could represent an innovative natural matrix rich in bioavailable and bioaccessible PACs for nutraceutical applications with potential beneficial properties.
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spelling pubmed-96573912022-11-15 A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability Iannuzzo, Fortuna Piccolo, Vincenzo Novellino, Ettore Schiano, Elisabetta Salviati, Emanuela Summa, Vincenzo Campiglia, Pietro Tenore, Gian Carlo Maisto, Maria Int J Mol Sci Article Proanthocyanidins (PACs) are a group of bioactive molecules found in a variety of plants and foods. Their bioavailability depends on their molecular size, with monomers and dimers being more bioavailable than those that have a higher polymerization degree. This study aimed to develop a method to convert high-molecular-weight PACs to low-molecular-weight ones in a grape seed extract (GSE) from Vitis vinifera L. Therefore, GSE was subjected to alkaline treatment (ATGSE), and its difference in chemical composition, compared to GSE, was evaluated using a molecular networking (MN) approach based on results obtained from HPLC-ESI HRMS/MS characterization analysis. The network analysis mainly noted the PAC cluster with about 142 PAC compounds identified. In particular, the obtained results showed a higher content of monomeric and dimeric PACs in ATGSE compared to GSE, with 58% and 49% monomers and 31% and 24% dimers, respectively. Conversely, trimeric (9%), polymeric (4%), and galloylated PACs (14%) were more abundant in GSE than in ATGSE (6%, 1%, and 4%, respectively). Moreover, in vitro antioxidant and anti-inflammatory activities were investigated, showing the high beneficial potential of both extracts. In conclusion, ATGSE could represent an innovative natural matrix rich in bioavailable and bioaccessible PACs for nutraceutical applications with potential beneficial properties. MDPI 2022-11-04 /pmc/articles/PMC9657391/ /pubmed/36362344 http://dx.doi.org/10.3390/ijms232113557 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Iannuzzo, Fortuna
Piccolo, Vincenzo
Novellino, Ettore
Schiano, Elisabetta
Salviati, Emanuela
Summa, Vincenzo
Campiglia, Pietro
Tenore, Gian Carlo
Maisto, Maria
A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title_full A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title_fullStr A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title_full_unstemmed A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title_short A Food-Grade Method for Enhancing the Levels of Low Molecular Weight Proanthocyanidins with Potentially High Intestinal Bioavailability
title_sort food-grade method for enhancing the levels of low molecular weight proanthocyanidins with potentially high intestinal bioavailability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9657391/
https://www.ncbi.nlm.nih.gov/pubmed/36362344
http://dx.doi.org/10.3390/ijms232113557
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