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Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins. Purification methods for recombinant proteins containing His-tags are relatively well-established, thus His-tags are widely used in pr...

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Detalles Bibliográficos
Autores principales: He, Shuzhen, Xu, Ruixian, Yi, Huashan, Chen, Zhixin, Chen, Congjie, Li, Qiang, Han, Qinqin, Xia, Xueshan, Song, Yuzhu, Xu, Junwei, Zhang, Jinyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658009/
https://www.ncbi.nlm.nih.gov/pubmed/36448055
http://dx.doi.org/10.1515/biol-2022-0521