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Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis
The split-Green Fluorescent Protein (GFP) reassembly assay is a powerful approach to study protein–protein interactions (PPIs). In this assay, two proteins, respectively, fused to the first seven and the last four β-strands of GFP are co-expressed in E. coli where they can bind to each other, which...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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MDPI
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658207/ https://www.ncbi.nlm.nih.gov/pubmed/36361946 http://dx.doi.org/10.3390/ijms232113167 |