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Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach

Grape seeds are the wineries’ main by-products, and their disposal causes ecological and environmental problems. In this study seeds from the pomace waste of autochthonous grape varieties from Lebanon, Obeidi (white variety) and Asswad Karech (red variety) were used for a multi-step biomass fraction...

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Autores principales: Salem, Yara, Rajha, Hiba N., van den Broek, Lambertus A. M., Safi, Carl, Togtema, Arnoud, Manconi, Maria, Manca, Maria Letizia, Debs, Espérance, Hobaika, Zeina, Maroun, Richard G., Louka, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658473/
https://www.ncbi.nlm.nih.gov/pubmed/36365284
http://dx.doi.org/10.3390/plants11212831
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author Salem, Yara
Rajha, Hiba N.
van den Broek, Lambertus A. M.
Safi, Carl
Togtema, Arnoud
Manconi, Maria
Manca, Maria Letizia
Debs, Espérance
Hobaika, Zeina
Maroun, Richard G.
Louka, Nicolas
author_facet Salem, Yara
Rajha, Hiba N.
van den Broek, Lambertus A. M.
Safi, Carl
Togtema, Arnoud
Manconi, Maria
Manca, Maria Letizia
Debs, Espérance
Hobaika, Zeina
Maroun, Richard G.
Louka, Nicolas
author_sort Salem, Yara
collection PubMed
description Grape seeds are the wineries’ main by-products, and their disposal causes ecological and environmental problems. In this study seeds from the pomace waste of autochthonous grape varieties from Lebanon, Obeidi (white variety) and Asswad Karech (red variety) were used for a multi-step biomass fractionation. For the first step, a lipid extraction was performed, and the obtained yield was 12.33% (w/w) for Obeidi and 13.04% (w/w) for Asswad Karech. For the second step, polyphenols’ recovery from the defatted seeds was carried out, resulting in 12.0% (w/w) for Obeidi and 6.6% (w/w) for Asswad Karech, with Obeidi’s extract having the highest total phenolic content (333.1 ± 1.6 mg GAE/g dry matter) and antioxidant activity (662.17 ± 0.01 µg/mL of Trolox equivalent). In the third step, the defatted and dephenolized seeds were subsequently extracted under alkaline conditions and the proteins were isoelectric precipitated. The recovered protein extract was 3.90% (w/w) for Obeidi and 4.11% (w/w) for Asswad Karech seeds, with Asswad Karech’s extract having the highest protein content (64 ± 0.2 mg protein/g dry matter). The remaining exhausted residue can be valorized in cosmetic scrubs formulations as a replacement for plastic microbeads. The designed zero-waste approach multi-step biomass fractionation has the potential to improve the valorization of the side products (grape seeds) of these two Lebanese autochthonous grape varieties.
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spelling pubmed-96584732022-11-15 Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach Salem, Yara Rajha, Hiba N. van den Broek, Lambertus A. M. Safi, Carl Togtema, Arnoud Manconi, Maria Manca, Maria Letizia Debs, Espérance Hobaika, Zeina Maroun, Richard G. Louka, Nicolas Plants (Basel) Article Grape seeds are the wineries’ main by-products, and their disposal causes ecological and environmental problems. In this study seeds from the pomace waste of autochthonous grape varieties from Lebanon, Obeidi (white variety) and Asswad Karech (red variety) were used for a multi-step biomass fractionation. For the first step, a lipid extraction was performed, and the obtained yield was 12.33% (w/w) for Obeidi and 13.04% (w/w) for Asswad Karech. For the second step, polyphenols’ recovery from the defatted seeds was carried out, resulting in 12.0% (w/w) for Obeidi and 6.6% (w/w) for Asswad Karech, with Obeidi’s extract having the highest total phenolic content (333.1 ± 1.6 mg GAE/g dry matter) and antioxidant activity (662.17 ± 0.01 µg/mL of Trolox equivalent). In the third step, the defatted and dephenolized seeds were subsequently extracted under alkaline conditions and the proteins were isoelectric precipitated. The recovered protein extract was 3.90% (w/w) for Obeidi and 4.11% (w/w) for Asswad Karech seeds, with Asswad Karech’s extract having the highest protein content (64 ± 0.2 mg protein/g dry matter). The remaining exhausted residue can be valorized in cosmetic scrubs formulations as a replacement for plastic microbeads. The designed zero-waste approach multi-step biomass fractionation has the potential to improve the valorization of the side products (grape seeds) of these two Lebanese autochthonous grape varieties. MDPI 2022-10-25 /pmc/articles/PMC9658473/ /pubmed/36365284 http://dx.doi.org/10.3390/plants11212831 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salem, Yara
Rajha, Hiba N.
van den Broek, Lambertus A. M.
Safi, Carl
Togtema, Arnoud
Manconi, Maria
Manca, Maria Letizia
Debs, Espérance
Hobaika, Zeina
Maroun, Richard G.
Louka, Nicolas
Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title_full Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title_fullStr Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title_full_unstemmed Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title_short Multi-Step Biomass Fractionation of Grape Seeds from Pomace, a Zero-Waste Approach
title_sort multi-step biomass fractionation of grape seeds from pomace, a zero-waste approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9658473/
https://www.ncbi.nlm.nih.gov/pubmed/36365284
http://dx.doi.org/10.3390/plants11212831
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