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Rapid Detection of Fusarium oxysporum Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol

We developed an insulated isothermal PCR (iiPCR) method for the efficient and rapid detection of Fusarium oxysporum (Fo), which is a fungus that infects various hosts and causes severe crop losses. The Fo iiPCR method was sensitive enough to detect up to 100 copies of standard DNA template and 10 fg...

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Detalles Bibliográficos
Autores principales: Chang, Tsai-De, Huang, Li-Nian, Lin, Yi-Jia, Wu, Zhong-Bin, Tsai, Shang-Han, Lin, Ying-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659253/
https://www.ncbi.nlm.nih.gov/pubmed/36362048
http://dx.doi.org/10.3390/ijms232113253