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Rapid Detection of Fusarium oxysporum Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol
We developed an insulated isothermal PCR (iiPCR) method for the efficient and rapid detection of Fusarium oxysporum (Fo), which is a fungus that infects various hosts and causes severe crop losses. The Fo iiPCR method was sensitive enough to detect up to 100 copies of standard DNA template and 10 fg...
Autores principales: | Chang, Tsai-De, Huang, Li-Nian, Lin, Yi-Jia, Wu, Zhong-Bin, Tsai, Shang-Han, Lin, Ying-Hong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9659253/ https://www.ncbi.nlm.nih.gov/pubmed/36362048 http://dx.doi.org/10.3390/ijms232113253 |
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