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Isolation of mouse mammary carcinoma-derived macrophages and cancer cells for co-culture assays
We recently established an in vitro co-culture system in which monophosphoryl lipid A + interferon-γ (MPLA+IFNγ)-treated tumor-associated macrophages (TAMs) killed cancer cells. Here, we describe a step-by-step protocol for isolating TAMs and cancer cells from mouse primary mammary carcinomas, the s...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664409/ https://www.ncbi.nlm.nih.gov/pubmed/36386879 http://dx.doi.org/10.1016/j.xpro.2022.101833 |