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Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts

Chinese chive (Allium tuberosum Rottler) is a popular food from Allium species in East and Southeast Asia. Most Allium species possess characteristic aromas and have antimicrobial activity. In this study, the antimicrobial activities of root, leaf, and scape extracts of Chinese chive at different pH...

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Autores principales: Chen, Cun, Cai, Jing, Ren, Ying-hong, Xu, Yue, Liu, Hong-ling, Zhao, Yu-yang, Chen, Xing-fu, Liu, Zhi-bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664698/
https://www.ncbi.nlm.nih.gov/pubmed/36386646
http://dx.doi.org/10.3389/fmicb.2022.1028627
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author Chen, Cun
Cai, Jing
Ren, Ying-hong
Xu, Yue
Liu, Hong-ling
Zhao, Yu-yang
Chen, Xing-fu
Liu, Zhi-bin
author_facet Chen, Cun
Cai, Jing
Ren, Ying-hong
Xu, Yue
Liu, Hong-ling
Zhao, Yu-yang
Chen, Xing-fu
Liu, Zhi-bin
author_sort Chen, Cun
collection PubMed
description Chinese chive (Allium tuberosum Rottler) is a popular food from Allium species in East and Southeast Asia. Most Allium species possess characteristic aromas and have antimicrobial activity. In this study, the antimicrobial activities of root, leaf, and scape extracts of Chinese chive at different pH levels (3.0, 5.0, 7.0, 9.0, and 10.7) were compared. The most pronounced activity was produced by the scape extract, and the greatest activity was obtained at pH 5.0. HPLC and GC–MS analysis showed that the major active ingredient was 2-amino-5-methylbenzoic acid. The mechanism of action of Chinese chive scape extracts may involve the depression or disruption of cell membrane integrity, according to our results of the leakage of electrolytes and protein, as well as scanning electron microscopy and transmission electron microscopy observations.
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spelling pubmed-96646982022-11-15 Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts Chen, Cun Cai, Jing Ren, Ying-hong Xu, Yue Liu, Hong-ling Zhao, Yu-yang Chen, Xing-fu Liu, Zhi-bin Front Microbiol Microbiology Chinese chive (Allium tuberosum Rottler) is a popular food from Allium species in East and Southeast Asia. Most Allium species possess characteristic aromas and have antimicrobial activity. In this study, the antimicrobial activities of root, leaf, and scape extracts of Chinese chive at different pH levels (3.0, 5.0, 7.0, 9.0, and 10.7) were compared. The most pronounced activity was produced by the scape extract, and the greatest activity was obtained at pH 5.0. HPLC and GC–MS analysis showed that the major active ingredient was 2-amino-5-methylbenzoic acid. The mechanism of action of Chinese chive scape extracts may involve the depression or disruption of cell membrane integrity, according to our results of the leakage of electrolytes and protein, as well as scanning electron microscopy and transmission electron microscopy observations. Frontiers Media S.A. 2022-11-01 /pmc/articles/PMC9664698/ /pubmed/36386646 http://dx.doi.org/10.3389/fmicb.2022.1028627 Text en Copyright © 2022 Chen, Cai, Ren, Xu, Liu, Zhao, Chen and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chen, Cun
Cai, Jing
Ren, Ying-hong
Xu, Yue
Liu, Hong-ling
Zhao, Yu-yang
Chen, Xing-fu
Liu, Zhi-bin
Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title_full Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title_fullStr Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title_full_unstemmed Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title_short Antimicrobial activity, chemical composition and mechanism of action of Chinese chive (Allium tuberosum Rottler) extracts
title_sort antimicrobial activity, chemical composition and mechanism of action of chinese chive (allium tuberosum rottler) extracts
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9664698/
https://www.ncbi.nlm.nih.gov/pubmed/36386646
http://dx.doi.org/10.3389/fmicb.2022.1028627
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