Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry

Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious. Here, we present a simple flow-cytometry-based protocol for quantitating T cell-APC contacts in the anti...

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Detalles Bibliográficos
Autores principales: Tatsumi, Naoya, Davila-Pagan, Alejandro, Kumamoto, Yosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667299/
https://www.ncbi.nlm.nih.gov/pubmed/36595900
http://dx.doi.org/10.1016/j.xpro.2022.101845

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667299/
https://www.ncbi.nlm.nih.gov/pubmed/36595900
http://dx.doi.org/10.1016/j.xpro.2022.101845

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