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Protocol to measure ß-galactosidase in Drosophila extracts using a CPRG assay

The quantification of ß-galactosidase activity is routinely required by laboratories worldwide. We present a cost-effective, highly replicable, simple technique for quantifying ß-galactosidase-specific activity from crude extracts made from whole organisms or dissected tissues or cells. Extracts are...

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Detalles Bibliográficos
Autores principales: Barwell, Taylor, Seroude, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667300/
https://www.ncbi.nlm.nih.gov/pubmed/36595888
http://dx.doi.org/10.1016/j.xpro.2022.101843