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Protocol to measure ß-galactosidase in Drosophila extracts using a CPRG assay
The quantification of ß-galactosidase activity is routinely required by laboratories worldwide. We present a cost-effective, highly replicable, simple technique for quantifying ß-galactosidase-specific activity from crude extracts made from whole organisms or dissected tissues or cells. Extracts are...
Autores principales: | Barwell, Taylor, Seroude, Laurent |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667300/ https://www.ncbi.nlm.nih.gov/pubmed/36595888 http://dx.doi.org/10.1016/j.xpro.2022.101843 |
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