Cargando…
Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia
Here, we provide a step-by-step protocol for generating human induced pluripotent stem cell (hiPSC)-based microglial mouse brain chimeras. In addition, we detail steps for intracerebral injection of pathological tau and magnetic cell isolation of human microglia from chimeric mouse brains for single...
| Autores principales: | , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667309/ https://www.ncbi.nlm.nih.gov/pubmed/36595906 http://dx.doi.org/10.1016/j.xpro.2022.101847 |