Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy

Dusquetide is a next-generation IDR (innate defense regulator) targeting the major autophagy receptor protein SQSTM1/p62 and modulating the innate immune response. Here, we describe a protocol for determining dusquetide-binding sites of p62 by solution NMR spectroscopy. Step-by-step technique detail...

Descripción completa

Detalles Bibliográficos
Autores principales: Penumutchu, Srinivasa, Liu, Jiuyang, Singh, Upendra K., Kutateladze, Tatiana G., Zhang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667315/
https://www.ncbi.nlm.nih.gov/pubmed/36595882
http://dx.doi.org/10.1016/j.xpro.2022.101842
_version_ 1784831699779059712
author Penumutchu, Srinivasa
Liu, Jiuyang
Singh, Upendra K.
Kutateladze, Tatiana G.
Zhang, Yi
author_facet Penumutchu, Srinivasa
Liu, Jiuyang
Singh, Upendra K.
Kutateladze, Tatiana G.
Zhang, Yi
author_sort Penumutchu, Srinivasa
collection PubMed
description Dusquetide is a next-generation IDR (innate defense regulator) targeting the major autophagy receptor protein SQSTM1/p62 and modulating the innate immune response. Here, we describe a protocol for determining dusquetide-binding sites of p62 by solution NMR spectroscopy. Step-by-step technique details were provided, including sample preparation, NMR experiment setup, data processing, and binding site analysis. This protocol could be applied to characterize other small molecules targeting the ZZ domain of p62 (9 kDa) or other proteins containing ZZ domains. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).
format Online
Article
Text
id pubmed-9667315
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-96673152022-11-17 Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy Penumutchu, Srinivasa Liu, Jiuyang Singh, Upendra K. Kutateladze, Tatiana G. Zhang, Yi STAR Protoc Protocol Dusquetide is a next-generation IDR (innate defense regulator) targeting the major autophagy receptor protein SQSTM1/p62 and modulating the innate immune response. Here, we describe a protocol for determining dusquetide-binding sites of p62 by solution NMR spectroscopy. Step-by-step technique details were provided, including sample preparation, NMR experiment setup, data processing, and binding site analysis. This protocol could be applied to characterize other small molecules targeting the ZZ domain of p62 (9 kDa) or other proteins containing ZZ domains. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022). Elsevier 2022-11-11 /pmc/articles/PMC9667315/ /pubmed/36595882 http://dx.doi.org/10.1016/j.xpro.2022.101842 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Penumutchu, Srinivasa
Liu, Jiuyang
Singh, Upendra K.
Kutateladze, Tatiana G.
Zhang, Yi
Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title_full Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title_fullStr Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title_full_unstemmed Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title_short Protocol to identify drug-binding sites in proteins using solution NMR spectroscopy
title_sort protocol to identify drug-binding sites in proteins using solution nmr spectroscopy
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667315/
https://www.ncbi.nlm.nih.gov/pubmed/36595882
http://dx.doi.org/10.1016/j.xpro.2022.101842
work_keys_str_mv AT penumutchusrinivasa protocoltoidentifydrugbindingsitesinproteinsusingsolutionnmrspectroscopy
AT liujiuyang protocoltoidentifydrugbindingsitesinproteinsusingsolutionnmrspectroscopy
AT singhupendrak protocoltoidentifydrugbindingsitesinproteinsusingsolutionnmrspectroscopy
AT kutateladzetatianag protocoltoidentifydrugbindingsitesinproteinsusingsolutionnmrspectroscopy
AT zhangyi protocoltoidentifydrugbindingsitesinproteinsusingsolutionnmrspectroscopy

Ejemplares similares