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Easily-controllable, helper phage-free single-stranded phagemid production system

BACKGROUND: Single-stranded (ss) DNAs are utilized in various molecular biological and biotechnological applications including the construction of double-stranded DNAs with a DNA lesion, and are commonly prepared by using chimeric phage-plasmids (phagemids) plus M13-derived helper phages. However, t...

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Detalles Bibliográficos
Autores principales: Suzuki, Tetsuya, Kamiya, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9667628/
https://www.ncbi.nlm.nih.gov/pubmed/36380394
http://dx.doi.org/10.1186/s41021-022-00254-1