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Protocol to purify recombinant inflammatory caspases and assess their catalytic activity in vitro

The inflammatory caspases, such as caspase-1, caspase-4, or caspase-11, are key enzymes in mammalian innate immunity as they control inflammasome-dependent inflammation. Assessing the specific proteolytic activities of these caspases in the context of a cell remains challenging, which is why in vitr...

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Detalles Bibliográficos
Autores principales: Devant, Pascal, Kagan, Jonathan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668568/
https://www.ncbi.nlm.nih.gov/pubmed/36595884
http://dx.doi.org/10.1016/j.xpro.2022.101848