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Protocol to purify recombinant inflammatory caspases and assess their catalytic activity in vitro
The inflammatory caspases, such as caspase-1, caspase-4, or caspase-11, are key enzymes in mammalian innate immunity as they control inflammasome-dependent inflammation. Assessing the specific proteolytic activities of these caspases in the context of a cell remains challenging, which is why in vitr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9668568/ https://www.ncbi.nlm.nih.gov/pubmed/36595884 http://dx.doi.org/10.1016/j.xpro.2022.101848 |