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RNA sensor response in HeLa cells for transfected mRNAs prepared in vitro by SP6 and HiT7 RNA polymerases: A comparative study

In vitro transcribed (IVT) synthetic mRNAs are in high demand due to their attractive bench to clinic translational processes. Mainly, the procedure to make IVT mRNA using bacteriophage RNA polymerases (RNAP) is relatively uncomplicated and scalable to produce large quantities in a short time period...

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Detalles Bibliográficos
Autores principales: Nagaraj, Siranjeevi, Stankiewicz-Drogon, Anna, Darzynkiewicz, Edward, Grzela, Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9669293/
https://www.ncbi.nlm.nih.gov/pubmed/36406230
http://dx.doi.org/10.3389/fbioe.2022.1017934