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Production and characterisation of modularly deuterated UBE2D1–Ub conjugate by small angle neutron and X-ray scattering

This structural study exploits the possibility to use modular protein deuteration to facilitate the study of ubiquitin signalling, transfer, and modification. A protein conjugation reaction is used to combine protonated E2 enzyme with deuterated ubiquitin for small angle X-ray and neutron scattering...

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Detalles Bibliográficos
Autores principales: Pietras, Zuzanna, Duff, Anthony P., Morad, Vivian, Wood, Kathleen, Jeffries, Cy M., Sunnerhagen, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9675693/
https://www.ncbi.nlm.nih.gov/pubmed/36289080
http://dx.doi.org/10.1007/s00249-022-01620-1